Physical and functional structure of a yeast plasmid, pSB3, isolated fromZygosaccharomyces bisporus
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (12), 4267-4283
- https://doi.org/10.1093/nar/13.12.4267
Abstract
The plasmid pSB3 of yeast Zygosacharomyces bisporus has been sequenced. It contains 6,615 base pairs, including a pair of inverted repeats (IR) consisting of 391 base pairs and 3 large open reading frames (ORF). One of the ORFs (A gene) participates in the recombination at the IRs and the other two (B and C genes) are necessary for the stable maintenance of this plasmid. The ARS sequence, which functions in a Saccharomyces cerevisiae host, was localized within 168 base pairs consisting of part of one of the IRs and a unique sequence contiguous to it. pSB3 can be maintained as stably in Z. rouxii as in the natural host Z.bisporus. In contrast, pSB3 is maintained fairly unstably in S.cerevisiae. The reason for this instability was found to be inefficient partitioning of pSB3 in S.cerevisiae. The molecular construction of pSB3 resembles that of 2-micron DNA, however, sequence homology at the DNA level was very poor.Keywords
This publication has 23 references indexed in Scilit:
- A rapid alkaline extraction procedure for screening recombinant plasmid DNANucleic Acids Research, 1979
- Isolation of a condensed, intracellular form of the 2-micrometer DNA plasmid of Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences, 1979
- Replication of each copy of the yeast 2 micron DNA plasmid occurs during the S phaseCell, 1979
- Functional expression of cloned yeast DNA in Escherichia coli: Specific complementation of argininosuccinate lyase (argH) mutationsJournal of Molecular Biology, 1978
- Control of Saccharomyces cerevisiae 2μm DNA replication by cell division cycle genes that control nuclear DNA replicationJournal of Molecular Biology, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- INHERITANCE OF 2-MU-M DNA PLASMID FROM SACCHAROMYCES1977
- Analysis of chromosomal integration and deletions of yeast plasmidsNucleic Acids Research, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Localization and Quantification of Circular DNA in YeastEuropean Journal of Biochemistry, 1974