Incorporation of atmospheric oxygen into the carbonyl functionality of the protochlorophyllide isocyclic ring
- 15 January 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 257 (2), 599-602
- https://doi.org/10.1042/bj2570599
Abstract
Detached cucumber (Cucumis sativus L. var. Beit Alpha) cotyledons incubated in darkness with 5-aminolaevulinic acid and either 16O2 air (control) or 18O2 in N2 accumulated protochlorophyllide. This was converted into methyl phaeoporphyrin alpha 5 and analysed by mass spectrometry. The molecular ion of the methyl phaeoporphyrin alpha 5 derived from the 18O2 incubation was 2 mass units greater than that of the control, establishing that the oxo oxygen atom of the isocyclic ring is derived from atmospheric oxygen.This publication has 15 references indexed in Scilit:
- THE MAGNESIUM-PROTOPROPHYRIN-IX (OXIDATIVE) CYCLASE SYSTEM - STUDIES ON THE MECHANISM AND SPECIFICITY OF THE REACTION SEQUENCE1988
- Intermediates in the Formation of the Chlorophyll Isocyclic RingPlant Physiology, 1985
- Properties of the Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase SystemPlant Physiology, 1985
- In Vitro Synthesis of the Chlorophyll Isocyclic RingPlant Physiology, 1982
- Chloroplast biogenesis. Detection of divinyl protochlorophyllide in higher plants.Journal of Biological Chemistry, 1980
- Reconstitution of chlorophyllide formation by isolated etioplast membranesBiochemical Journal, 1978
- Effect of the Hypocotyl Hook on Greening in Etiolated Cucumber CotyledonsPlant Physiology, 1970
- The breakdown of chlorophyll by chlorophyllaseBiochemical Journal, 1961
- THE SUCCINATE-GLYCINE CYCLE .1. THE MECHANISM OF PYRROLE SYNTHESIS1955
- THE MECHANISM OF PORPHYRIN FORMATION - FURTHER EVIDENCE ON THE RELATIONSHIP OF THE CITRIC ACID CYCLE AND PORPHYRIN FORMATION1955