DNA stretching on functionalized gold surfaces
Open Access
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (3), 492-497
- https://doi.org/10.1093/nar/22.3.492
Abstract
We describe a method for anchoring bacteriophage λ DNA by one end to gold by Au - blotin - streptavidin - blotin - DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned.Keywords
This publication has 20 references indexed in Scilit:
- Atomic force microscopy of DNA and bacteriophage in air, water and propanol: the role of adhesion forcesNucleic Acids Research, 1993
- Direct Mechanical Measurements of the Elasticity of Single DNA Molecules by Using Magnetic BeadsScience, 1992
- Estimating Genomic Distance from DNA Sequence Location in Cell Nuclei by a Random Walk ModelScience, 1992
- Circular DNA molecules imaged in air by scanning force microscopyBiochemistry, 1992
- Magnetic separation of DNANature, 1989
- Observation of Individual DNA Molecules Undergoing Gel ElectrophoresisScience, 1989
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984
- Immunological method for mapping genes on Drosophila polytene chromosomes.Proceedings of the National Academy of Sciences, 1982
- Yeast Chromosomal DNA: Size, Structure, and ReplicationCold Spring Harbor Symposia on Quantitative Biology, 1974
- The bacterial chromosome and its manner of replication as seen by autoradiographyJournal of Molecular Biology, 1963