Titin: Quantitative mass measurements by scanning transmission electron microscopy and structural implications for the sarcomere matrix of skeletal muscle
- 4 July 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 234 (1), 145-148
- https://doi.org/10.1016/0014-5793(88)81321-8
Abstract
Scanning transmission electron microscopy has been used to investigate mass and linear mass density of native titin-2, a large soluble fragment of intact titin, from rabbit skeletal muscle. Dark field images of unstained, freeze-dried titin-2 appeared as either compact globules or looser and larger balls of string. Direct mass measurements indicated that the compact forms have an average mass of 2.40±0.50 × 106 Da. The mass to length ratio, determined from well-spread portions of titin strands (3–5 nm wide) from the ball of string forms, averaged 2.7±0.9 kDa/nm. Thus a single native intact titin molecule has a calculated contour length of well above ∼1 μm, sufficient to span unidirectionally between the Z line and M line region in a resting-length sarcomere.Keywords
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