Effect of Temperature on Ionographic Mobilities in Paper Stabilized Media.

Abstract

The effect of temperature on velocity of electromigration through paper stabilized electrolytes, of a variety of substances, including bromphenol blue, bovine plasma albumin, human serum albumin and globulin fractions and human serum lipoproteins, was investigated. The ionographic apparatus employed utilized horizontal paper strips in a water saturated atmosphere. The runs were made at temperatures from 4[degree]C to 25[degree]C and under constant conditions of buffer, ionic strength, pH and potential gradient. For all substances studied, the relationship between the mobility and temperature can be represented by the equation log (mobility) = m . 1/T + B where T is [degree]Kelvin, B is a constant and m is the slope of the line obtaines when the logarithm of the mobility is plotted against the reciprocal of the absolute temperature.