Nucleotide Sequence of Part of the Simian Virus 40 Hind‐D Restriction Fragment

Abstract

The nucleotide sequence at the beginning of the restriction fragment Hind-D from SV-40 DNA was derived by partial chemical degradation of (5''-32P)-labeled restriction fragments followed by analysis on polyacrylamide gel. The sequence reported here is 140 nucleotides long. It contains an ATG codon which presumably corresponds to the initiator codon of the VP2 protein. This codon is preceded by an untranslated region which shows several interesting features, such as an alternation of (dA + dT)-rich and (dG + dC)-rich blocks.