The Regulation of Glycolate Metabolism in Division Synchronized Cultures of Euglena
Open Access
- 1 May 1971
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 47 (5), 640-643
- https://doi.org/10.1104/pp.47.5.640
Abstract
Phosphoglycolate and phosphoglycerate phosphatases and glycolate dehydrogenase activities were determined in division synchronized Euglena gracilis strain Z cultures. Phosphoglycolate phosphatase activity remained nearly constant in the light but doubled in the dark, whereas phosphoglycerate phosphatase activity decreased by half in the light and increased 4-fold over the dark phase of the cycle. Glycolate dehydrogenase activity assayed by dye reduction increased over the light and remained constant during the dark phase, but when determined by the phenylhydrazone method, an assay dependent upon the presence of a natural hydrogen acceptor, activity decreased in the dark phase. The acceptor decayed in the dark in all cell-free extracts and 3-(3,4-dichlorophenyl)-1, 1-dimethylurea inhibited light regeneration.Keywords
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