Molecular diagnostics using analytical immuno high performance liquid affinity chromatography
- 1 September 1987
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 16 (1), 119-128
- https://doi.org/10.1007/bf02798361
Abstract
Analytical immuno high performance liquid affinity chromatography (analytical immuno HPLAC) was evaluated as a molecular diagnostic tool. Antibodies raised in rabbits against bovine neurophysin II were immobilized through Protein A crosslinking onto coated silica. Interaction of immobilized antibody with mobile antigen was characterized by zonal and frontal elutions of14C-labeled bovine neurophysin II under isocratic, nondenaturing conditions. The Chromatographic behavior shows that analytical immuno HPLAC with immobilized antibodies can be used to detect the number and functional nature of matrix-interacting antigens in mixtures, thus providing a quantitative Chromatographic technology for “antigen mapping”.Keywords
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