Delayed-Type Hypersensitivity as Revealed on the Footpads of Mice to Azobenzenearsonate-Acetyl Bovine Serum Albumin

Abstract

A strong delayed-type footpad reaction was established in mice using azobenzenearsonate-acetyl bovine serum albumin (ABA-AcBSA) as an antigen. Male ddY/S mice were sensitized by subcutaneous injection of 100 μl of Freund’s complete adjuvant – saline (1:1) emulsion containing 100 μg of the antigen and challenged by subcutaneous injection of 2.5 μl of Freund’s incomplete adjuvant – saline (1:1) emulsion with 2.5 μg antigen in the footpad on the 10th day after the sensitization. The analysis of the system which fulfilled criteria for delayed-type hypersensitivity with regard to kinetics, passive transfer and histology of the footpad reaction, and the effect of dexamethasone, indomethacin and quinacrine on the footpad reaction were described.