High-sensitivity radioimmunoassay for substance P
- 1 December 1976
- journal article
- Published by Springer Nature in Irish Journal of Medical Science
- Vol. 145 (1), 392-398
- https://doi.org/10.1007/bf02938978
Abstract
Optimisation of a non-equilibrium radioimmunoassay for substance P is described. The assay has a sensitivity of 2 pg (1.5 fmole), sufficient for substance P determinations in unexcepted human plasma and other biological material. The specificity of antisera used was tested with closely related peptides, physalaemin and eledoisin. Crossreaction of 1 per cent for eledoisin and 0.1 per cent for physalaemin was found. The assay is easy to perform, has a precision of 5 per cent, and takes only 24 hours.Keywords
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