Studies on the Serum Proteins of Chimeras I.

Abstract

Rat-into-mouse chimeras were produced by injection of rat bone marrow into irradiated mice. The serum proteins of these chimeras and their sites of production were studied using extensively absorbed antisera specific for rat or mouse serum proteins. Incorporation of C14- amino acid into serum proteins, identified by autoradiography of immuno-electrophoretic patterns from tissue culture fluids was used to demonstrate synthesis in vitro. It was shown that rat transferrin and [beta]1C were produced by spleen and peritoneal macrophages, and rat immune globulins, gamma1, gamma2 and IgM, by spleen and mesenteric lymph node. Production of transferrin and [beta]1C preceded that of immune globulins. Liver tissue produced mouse serum proteins only.