Teratogenic effects of combined administration of ethylenethiourea and nitrite in mice

Abstract
Teratogenic potential of ethylenethiourea (ETU) was investigated in SLC‐ICR mice after its reaction with sodium nitrite. ETU was given orally in dose of 400 mg/kg on various days of pregnancy in combination with 200 mg/kg NaNO2 at varied intervals. When NaNO2 was given to females immediately after their treatment with ETU on day 6 or 8 of pregnancy, fetal survival was significantly decreased. Various types of malformations were observed in the living fetuses from mothers treated on day 6, 8, or 10 of pregnancy, but not on day 12. The teratogenicity disappeared when NaNO2 was given 2 h after the treatment with ETU.