Methods for the Analysis and Characterization of Forensic Samples Containing Amphetamines and Related Amines

Abstract

Liquid chromatographic methods are described for the analysis of forensic samples containing amphetamine and methamphetamine. These are among the more common drugs encountered from clandestine laboratories. The methods are illustrated with numerous examples of drugs submitted for forensic analysis. Precolumn derivatization of these amines with phenylisothiocyanate yields thiourea products having good reversed-phase chromatographic properties and high UV molar absorptivities. Using similar derivatization procedures, the enantiomeric composition can be determined for those amines containing a chiral center. The separation of the diastereolsomeric products of chiral derivatization can be accomplished using standard reversedphase conditions on achiral C₁₈ stationary phases. These procedures allow the forensic chemist to determine the identity and stereochemistry of amphetamines and related compounds in forensic samples.