Rat Brain Glutamic Acid Decarboxylase Sequence Deduced from a Cloned cDNA
- 1 February 1990
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 54 (2), 703-705
- https://doi.org/10.1111/j.1471-4159.1990.tb01928.x
Abstract
A cDNA clone complementary to the rat brain glutamic acid decarboxylase mRNA was isolated from a rat brain cDNA expression library using an antibody specific to the enzyme. The cDNA insert has been shown to direct the synthesis of an active protein in Escherichia coli. In this study, the nucleotide sequence of this clone, which includes the complete coding region, is presented. The predicted protein is 593 amino acids in length. The first 557 residues display a 95% identity when compared with the corresponding cat sequence. However, the deduced amino acid sequence of the carboxy-terminal end of the rat protein, downstream of residue 557, is totally different from the cat, whereas it agrees with a published partial peptidic sequence of the rat protein.Keywords
This publication has 16 references indexed in Scilit:
- Pyridoxal 5'-phosphate dependent histidine decarboxylase: overproduction, purification, biosynthesis of soluble site-directed mutant proteins and replacement of conserved residuesBiochemistry, 1989
- Molecular cloning and analysis of cDNA encoding a plant tryptophan decarboxylase: comparison with animal dopa decarboxylases.Proceedings of the National Academy of Sciences, 1989
- Pattern of expression of glutamic acid decarboxylase mRNA in the developing rat brain.Proceedings of the National Academy of Sciences, 1988
- Molecular cloning, expression and in situ hybridization of rat brain glutamic acid decarboxylase messenger RNANeuroscience Letters, 1987
- Brain Glutamate Decarboxylase Cloned in λgt-11: Fusion Protein Produces γ-Aminobutyric AcidScience, 1986
- Phylogenesis of Brain Glutamic Acid Decarboxylase from Vertebrates: Immunochemical StudiesJournal of Neurochemistry, 1986
- Use of statistical criteria for screening potential homologies in nucleic acid sequencesNucleic Acids Research, 1984
- [2] New M13 vectors for cloningMethods in Enzymology, 1983
- Production of a specific antiserum to rat brain glutamic acid decar☐ylase by injection of an antigen-antibody complexNeuroscience, 1981
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977