A Rapid Filter Paper Disk Assay for Picomole Amounts of Cyclic AMP Using a Cyclic AMP Dependent Protein Kinase

Abstract

A rapid filter paper disk assay for cyclic AMP based on the activation of a cyclic AMP dependent protein kinase from rabbit skeletal muscle is described. This procedure permits determination of as little as 0.2 picomoles of cyclic AMP. In addition this procedure employs ATP-γ-³³P instead of ATP- γ-³²P. 1 This work was performed while the author was a USPHS postdoctoral fellow in the laboratory of Dr. Van R. Potter. Financial support was provided by departmental Grant CA 07175, Training Grant T01-Ca5002, and a USPHS postdoctoral fellowship 5 F02-CA-43,880-02 through the National Cancer Institute.