RESOLVING POWER, SENSITIVITY AND LATENT IMAGE FADING OF SOLUBLE-COMPOUND AUTORADIOGRAPHS

Abstract

The resolving power, sensitivity and latent image fading have been studied in autoradiographs prepared by a technique that retains soluble compounds. A resolution of 2-4 µ was obtained in preparations containing ³H-thymidine and 9-13 µ in those containing ²²NaCl. Results showed that there was no significant diffusion of soluble compounds. Sensitivity studies revealed that –25°C was the optimum exposure temperature. There was no significant latent image fading in preparations exposed for periods up to 330 days.