BARHL2 Differentially Regulates the Development of Retinal Amacrine and Ganglion Neurons
Open Access
- 1 April 2009
- journal article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 29 (13), 3992-4003
- https://doi.org/10.1523/jneurosci.5237-08.2009
Abstract
Through transcriptional regulations, the BarH family of homeodomain proteins play essential roles in cell fate specification, cell differentiation, migration, and survival. Barhl2, a member of the Barh gene family, is expressed in retinal ganglion cells (RGCs), amacrine cells (ACs), and horizontal cells. Here, to investigate the role of Barhl2 in retinal development, Barhl2-deficient mice were generated. Analysis of AC subtypes in Barhl2-deficient retinas suggests that Barhl2 plays a critical role in AC subtype determination. A significant reduction of glycinergic and GABAergic ACs with a substantial increase in the number of cholinergic ACs was observed in Barhl2-null retinas. Barhl2 is also critical for the development of a normal complement of RGCs. Barhl2 deficiency resulted in a 35% increase in RGCs undergoing apoptosis during development. Genetic analysis revealed that Barhl2 functions downstream of the Atoh7–Pou4f3 regulatory pathway and regulates the maturation and/or survival of RGCs. Thus, BARHL2 appears to have numerous roles in retinal development, including regulating neuronal subtype specification, differentiation, and survival.Keywords
This publication has 46 references indexed in Scilit:
- Bhlhb5 Regulates the Postmitotic Acquisition of Area Identities in Layers II-V of the Developing NeocortexNeuron, 2008
- ISL1 and BRN3B co-regulate the differentiation of murine retinal ganglion cellsDevelopment, 2008
- The LIM-Homeobox GeneIslet-1Is Required for the Development of Restricted Forebrain Cholinergic NeuronsJournal of Neuroscience, 2008
- Islet-1 Controls the Differentiation of Retinal Bipolar and Cholinergic Amacrine CellsJournal of Neuroscience, 2007
- Requirement for Bhlhb5 in the specification of amacrine and cone bipolar subtypes in mouse retinaDevelopment, 2006
- Diversity of ganglion cells in the mouse retina: Unsupervised morphological classification and its limitsJournal of Comparative Neurology, 2005
- Proneural genes and the specification of neural cell typesNature Reviews Neuroscience, 2002
- The fundamental plan of the retinaNature Neuroscience, 2001
- Neuronal specification in the spinal cord: inductive signals and transcriptional codesNature Reviews Genetics, 2000
- Regulation of a Segmentation Stripe by Overlapping Activators and Repressors in the Drosophila EmbryoScience, 1991