Trypanosoma cruzi: Quantification and Analysis of the Infectivity of Cloned Stocks1

Abstract
The infection of bovine embryo skin and muscle cells by trypomastigotes of 4 T. cruzi clones (CA-I/71, /72, Miranda/76, /80) was quantified. Stable and reproducible intraisolate differences were observed; an almost 70-fold difference in infectivity occurred between clones. The CA-I/71 clone was not susceptible to N-acetyl-D-glucosamine at a concentration that inhibits the infection of vertebrate cells by Ernestina and Y-strain parasites. Eight other monosaccharides that are common constituents of vertebrate cell surface glycoproteins also failed to inhibit the infection of vertebrate cells by the CA-I/71 clone.