Cell Separations on the Countercurrent Chromatograph
- 1 February 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (2), 419-431
- https://doi.org/10.1080/01483918408073976
Abstract
Separation of cells differing only subtly has been achieved by partitioning between the two phases formed by solution of dextran and polyethylene glycol in water. Cell populations which have related, but not identical, surface properties seldom exhibit sufficiently different partition behavior to be separated in a single extraction. In such cases, repeated partitions are carried out via countercurrent distribution or countercurrent chromatography to effect the separation. Potential advantages of countercurrent chromatography are its ease and rapidity of operation. In the present work we describe our approach to determining and possibly improving the efficiency of polymer phase partitioning by countercurrent chromatography.This publication has 3 references indexed in Scilit:
- Improved Nonsynchronous Flow-Through Coil Planet Centrifuge without Rotating Seals: Principle and ApplicationSeparation Science and Technology, 1983
- Cell separation using two-phase polymer systems in a nonsynchronous flow-through coil planet centrifugeAnalytical Biochemistry, 1980
- Nonsynchronous flow-through coil planet centrifuge applied to cell separation with physiological solutionAnalytical Biochemistry, 1979