Simplified template preparation and improved direct sequencing using Taq polymerase.
Open Access
- 1 February 1992
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 1 (4), 255-256
- https://doi.org/10.1101/gr.1.4.255
Abstract
A streamlined version of direct dideoxy sequencing is presented that includes template preparation as well as sequencing protocols. The method is used routinely to sequence double-stranded PCR products after minimal purification with one of the primers used in amplification. Either 35S or 32P labeling can be used with equally good results.Keywords
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