Reduction of Nitrate and Nitrite by Subcellular Preparations of Anabaena cylindrica. I. Reduction of Nitrite to Ammonia
Open Access
- 1 June 1966
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 41 (6), 1031-1036
- https://doi.org/10.1104/pp.41.6.1031
Abstract
Reduction of nitrite by cell-free preparations of Anabaena cylindrica in the dark has been investigated. Nitrite-reducing activity was recovered in a supernatant fraction. The nitrite reductase system was partially purified by column chromatography on Sephadex G-75. NADPH could serve as an H-donor. NADH was completely inactive. The reduction required ferredoxin which mediated the transfer of electrons from NADPH to nitrite. Ferredoxin was successfully replaced with methyl viologen, benzyl viologen and diquat. The nitrite-reducing activity was inhibited by KCN, and by 2,4-dinitrophenol and arsenate at higher concentrations. The extent of nitrite reduction by NADPH was dependent on the oxidation-reduction states of NADP and ferredoxin.Keywords
This publication has 4 references indexed in Scilit:
- Light and Dark Reduction of Nitrite in a Reconstituted Enzymic SystemJournal of Biological Chemistry, 1964
- Enzymic Assimilation of Nitrate in Tomato Plants. II. Reduction of Nitrite to AmmoniaPlant Physiology, 1964
- PYRIDINE NUCLEOTIDE TRANSHYDROGENASE FROM SPINACH .1. PURIFICATION AND PROPERTIES1960
- The enzymic reduction of nitrite to ammonia by reduced pyridine nucleotidesBiochimica et Biophysica Acta, 1954