Microdensitometry with image analyser video scanners
- 1 March 1981
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 121 (3), 337-346
- https://doi.org/10.1111/j.1365-2818.1981.tb01229.x
Abstract
A numerical method for calibration of the Quantimet 720 1D Auto detector as a densitometer in absolute density units was developed and applied to integrated microdensitometric measurement. A procedure for the estimation and correction of microscope glare error was also derived. The error after glare correction was comparable with threshold setting variation encountered in routine use of the Quantimet. The method should prove valuable in densitometric applications not only for Quantimet image analyzers but also for any instrument employing a video imaging system because the underlying principles are the same.This publication has 7 references indexed in Scilit:
- Petrographic Characterization Of Coal Using Automatic Image AnalysisJournal of Microscopy, 1979
- Principles and methods for the quantitative determination of feulgen stained DNA with the television texture analysis system (TAS)Histochemistry and Cell Biology, 1979
- Apparent anomalies in nuclear feulgen-DNA contents. Role of systematic microdensitometric errors.The Journal of cell biology, 1976
- Area representation of optical density of chromatin in resting and stimulated lymphocytes as measured by means of quantimetHistochemistry and Cell Biology, 1972
- Aspects of scanning microdensitometry I. Stray light (glare)Journal of Microscopy, 1970
- Das Quantimet als integrierendes Mikrophotometer / The Quantimet as an Integrating MicrophotometerPractical Metallography, 1969
- Erfassung morphologischer Merkmale von Zellkernen mittels bildanalytischer Meßdaten / Recognition of Morphological Features of Cell Nuclei Using Image AnalysisPractical Metallography, 1969