MICROBIOLOGICAL STUDIES ON PETROLEUM AND NATURAL GAS XIII. IDENTIFICATION OF THE FLAVIN NUCLEOTIDE-FORMING BACTERIA

Abstract

A comparative experiment was carried out on the 60 taxonomical characteristics for the hydrocarbon-utilizing and flavin nucleotide-forming bacteria (strain S5, and new isolates S204 and S2012), and the type strain IAM 12087 (ATCC 23055) and an authentic strain IAM 12088 (ATCC 19606) of Acinetobacter calcoaceticus. Its results showed a similarity value of 85% and more among the isolates and other strains. Guanine plus cytosine content of DNA of these strains was 41 or 42%, which showed the values of Acinetobacter spp. From these results, the 3 isolates are identified as A. calcoaceticus according to Bergey''s Manual of Determinative Bacteriology, Eighth Edition, but these isolates were named A. anitratum (Schaub and Hauber) Brisou and Prevot 1954. The name A. anitratum is more suitable to use as a type species name instead of A. calcoaceticus according to the International Code of Nomenclature of Bacteria. It seemed that the flavin pigmentation (FMN and FAD) was 1 of the specificities of Acinetobacter spp.