Enzymatic activity of the ribosome‐bound nascent polypeptide
- 8 January 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 378 (2), 166-170
- https://doi.org/10.1016/0014-5793(95)01438-1
Abstract
Firefly luciferase was shown to be completely folded and thus enzymatically active immediately upon release from the ribosome [Kolb et al. (1994) EMBO J. 13, 3631-3637]. However, no luciferase activity was observed while full-length luciferase was attached to the ribosome as a peptidyl-tRNA, probably because the C-terminal portion of the enzyme is masked by the ribosome and/or ribosome-associated proteins. Here we have demonstrated that the ribosome-bound enzyme acquires the enzymatic activity when its C-terminus is extended by at least 26 additional amino acid residues. The results demonstrate that the acquisition of the final native conformation by a nascent protein does not need the release of the protein from the ribosome.Keywords
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