MASS PREPARATION OF NUCLEI FROM THE LARVAL SALIVARY GLANDS OF DROSOPHILA HYDEI
Open Access
- 1 August 1968
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 38 (2), 369-376
- https://doi.org/10.1083/jcb.38.2.369
Abstract
A method has been developed for isolating gram quantities of salivary glands from late third instar larvae of Drosophila hydei. The isolated glands have a normal appearance and incorporate RNA and DNA precursors normally. Nuclei can be isolated from these glands in 90% yield with the use of detergents. These nuclei contain morphologically normal giant polytene chromosomes.Keywords
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