A Simple Method for Preservation of Fine Structure in Blood Cells
- 31 December 1966
- journal article
- research article
- Published by Georg Thieme Verlag KG in Thrombosis and Haemostasis
- Vol. 18 (03/04), 745-753
- https://doi.org/10.1055/s-0038-1655084
Abstract
A technique is described which simplifies the preparation of blood leukocytes and platelets for electron microscopy without sacrificing morphologic preservation. The method has the following advantages : 1. Simplicity of procedure without sacrifice of morphology. 2. Elimination of need for rapid processing and cumbersome ice water baths. 3. Samples may be shipped to other laboratories after initial fixation. 4. Applicable to cytochemical and immunochemical studies. 5. Fine structural preservation is excellent. Structures which are destroyed by cold are maintained by the method described. * Associate Professor, Department of PediatricsFunding Information
- Cardiovascular Clinical Research Program Project, Graduate School of the University of Minnesota, and the American Cancer Society, Minnesota Division (USPHS #AM 02917 and #AI 05153)