The metabolism of [1- 13 C]-glucose by Paracoccus denitrificans , investigated by gas chromatography-mass spectrometry

Abstract

The metabolism of P. denitrificans, grown on [1-13C]-glucose as the sole C source, was investigated by using gas chromatography-mass spectrometry and metabolic profiling. The distribution of label in a wide range of metabolites (42 in total) showed that the Embden-Meyerhoff pathway did not operate in vivo, whereas the Entner-Doudoroff and pentose phosphate pathways were operative under the specified growth conditions. Of the [1-13C]-glucose, 31% was metabolized by the Entner-Doudoroff pathway and 69% by the pentose phosphate pathway.