Radionuclide levels in fish from the Beaverlodge Lake area, Saskatchewan, Canada, varied significantly among lakes, species and tissues, but did not vary with fish sex or age. Fish from lakes affected by an operating uranium mine and mill had radionuclide levels one to two orders of magnitude above levels in fish from an uncontaminated control lake. Concentrations were greatest in white suckers, intermediate in lake whitefish, and low in lake trout. Skin and bone contained the highest radionuclide levels; levels in flesh were much lower. The mean ash-weight concentration of 226Ra in non-control, eviscerated, whole fish was 9.3, 1.8 and 0.8 pCi * g−1 in white suckers, lake whitefish and lake trout respectively. Mean 210Pb levels for those species were 4.0, 1.4 and 0.5 pCi * g−1, while U content was 96.8, 28.2 and 5.8 μg * g−1, respectively. Radium-226 content in forage fish ranged from 9.7–70 pCi * g−1. Lead-210 levels ranged from 3.9–55.0 pCi * g−1, while U levels ranged from 15–119 μg * g−1. Bioconcentration factors varied among sites, species and tissues. The primary radionuclide transfer pathway appears to be via sediments, either through direct contact or through food organisms. Bottom-feeding species had the highest radionuclide levels while piscivorous fish had the lowest levels. The internal dose rate to non-control fish are comparatively low; any effects are likely to occur at the genetic level.