Functional Nonequivalence of α and β Hemes in Human Adult Hemoglobin
- 1 July 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (7), 1707-1710
- https://doi.org/10.1073/pnas.69.7.1707
Abstract
Nuclear magnetic resonance studies of the contact-shifted spectra of heme protons in deoxyhemoglobin A from human adults show conclusively that oxygen binds to the alpha hemes in preference to the beta hemes. The preferential binding is produced in 10% hemoglobin solution at neutral pH by either a 15-fold molar excess of 2,3-diphosphoglycerate or a 5-fold molar excess of inositol hexaphosphate. Preferential binding is not observable in the absence of the organic phosphates. The results indicate that the oxygenation of hemoglobin may be described by a sequential model, or by a concerted model that allows the alpha hemes to bind ligand first.Keywords
This publication has 21 references indexed in Scilit:
- Mechanism of Cooperative Oxygen Binding to HemoglobinProceedings of the National Academy of Sciences, 1972
- An allosteric model of hemoglobin: I, kineticsJournal of Molecular Biology, 1971
- Interaction of haemoglobin with ions binding of inositol hexaphosphate to human haemoglobin aFEBS Letters, 1971
- The reaction of n-butyl isocyanide with human hemoglobin. I. Determination of the kinetic parameters involved in the last step in ligand binding.1971
- Effect of inositol hexaphosphate and other organic phosphates on the cooperativity in oxygen binding of human hemoglobinsBiochemical and Biophysical Research Communications, 1971
- The Binding of Carbon Monoxide to α and β Chains in Tetrameric Mammalian HemoglobinJournal of Biological Chemistry, 1971
- The removal of organic phosphates from hemoglobinArchives of Biochemistry and Biophysics, 1971
- Observation of allosteric transition in hemoglobinBiochemical and Biophysical Research Communications, 1971
- Nuclear magnetic resonance studies of hemoglobins. V. The heme proton spectra of human deoxyhemoglobins A, F, Zürich, and ChesapeakeBiochemical and Biophysical Research Communications, 1970
- On the nature of allosteric transitions: A plausible modelJournal of Molecular Biology, 1965