TURNOVER RATES OF MUSCLE PROTEIN IN CARDIAC AND SKELETAL MUSCLES OF DOG, FOWL, RAT AND MOUSE: TURNOVER RATE RELATED TO MUSCLE FUNCTION

Abstract

Turnover rates of muscle protein in cardiac and skeletal muscle have been measured in dogs using a continuous infusion of ¹⁴C-tyrosine, and in fowls, rats and mice after a pulse label of either ³H-leucinc or ³H-aspartate. In all species the total mixed protein from ventricle turned over the most rapidly. Tonic muscles predominantly comprised of red fibres were Intermediate in rate and twitch muscles comprised of predominantly white fibres had the slowest rates of turnover. This same progression of turnover rates was also seen in myofibrillar and sarcoplasmic protein fractions and with myosin. The RNA concentrations of the muscle tissue were highly correlated with the turnover rates. It is suggested that the turnover rate of muscle protein is related to the function of the muscle.