Skeletal muscle glutamine production in thermally injured rats
- 1 February 1988
- journal article
- research article
- Published by Portland Press Ltd. in Clinical Science
- Vol. 74 (2), 165-172
- https://doi.org/10.1042/cs0740165
Abstract
1. The effect of thermal injury (33-35% of body surface area) on the regulation of glutamine metabolism was studied in skeletal muscles of rats 7 days after injury. 2. Injury increased the rates of glutamine production in muscle, skin and adipose tissue preparations, with muscle production accounting for over 90% of total glutamine produced by the hindlimb. 3. Injury produced decreases in the concentrations of skeletal muscle glutamine (36%, P < 0.001), glutamate (39%, P < 0.001), alanine (24%, P < 0.001), pyruvate (35%, P < 0.001), 2-oxoglutarate (51%, P < 0.001) and adenosine 5''-triphosphate, (38%, P < 0.001). The concentrations of ammmonia (42%, P < 0.001) were increased. 4. The maximal activity of glutamine synthetase was increased (22-40%, P < 0.001) in muscles of injured rats, whereas that of glutaminase was unchanged. 5. Hindlimb blood flow decreased by approximately 15% in injured rats, which was accompanied by an enhanced net release of glutaminase (80%, P < 0.001) and alanine (44%, P < 0.001). 6. It is concluded that there is an enhanced rate of release of both glutamine and alanine from skeletal muscle of thermally injured rats. This may be due to changes in efflux and/or increased intracellular formation of glutamine and alanine.This publication has 24 references indexed in Scilit:
- The Effect of Burn Wound Size on Ureagenesis and Nitrogen BalanceAnnals of Surgery, 1982
- Plasma and Skeletal Muscle Amino Acids Following Severe Burn Injury in Patients and Experimental AnimalsAnnals of Surgery, 1982
- Amino acid release from the hindquarter and urea appearance in acute uremiaAmerican Journal of Physiology-Endocrinology and Metabolism, 1981
- Ammonia partitioning between glutamine and urea: Interorgan participation in metabolic acidosisKidney International, 1981
- Muscle protein degradation and amino acid metabolism in human injuryBiochemical Society Transactions, 1980
- Muscle and Plasma Amino Acids Following Injury Influence of Intercurrent InfectionAnnals of Surgery, 1980
- Development and analysis of a small animal model simulating the human postburn hypermetabolic responseJournal of Surgical Research, 1978
- ARTERIAL PLASMA AMINO-ACIDS IN PATIENTS WITH SEVERE BURNS1978
- AMINO ACID EXTRACTION AND AMMONIA METABOLISM BY THE HUMAN KIDNEY DURING THE PROLONGED ADMINISTRATION OF AMMONIUM CHLORIDE*JCI Insight, 1963
- The amino acid composition of actin, myosin, tropomyosin and the meromyosinsArchives of Biochemistry and Biophysics, 1954