Enantiomeric HPLC Separation of Selected Chiral Drugs Using Native and Derivatized β-Cyclodextrins as Chiral Mobile Phase Additives

Abstract

Native β-Cyclodextrin and three of its derivatives, hydroxypropyl, methyl and sulfated β-cyclodextrins were used as chiral mobile phase additives to investigate the enantiomeric separation of selected drugs by reversed phase HPLC. The chiral drugs investigated were oxazepam, temazepam, lorazepam, ketoprofen, fenoprofen, ibuprofen, chlorthalidone, terbutaline, trimeprazine and trimipramine. Different types of reversed phase columns were investigated including propylsilane, hexylsilane, octylsilane, octadecylsilane columns and a new nonporous octadecylsilane column. The new nonporous column gave separations for most of the drugs including short retention times. Terbutaline was not separated on the nonporous column, but did separate on hexylsilane and octylsilane columns. The effect of various mobile phases, organic modifier, type and concentration of the added cyclodextrin on peak shape, resolution and retention factors of the enantiomers were investigated.