High resolution proton NMR investigations of rat blood plasma Assignment of resonances for the molecularly mobile carbohydrate side‐chains of ‘acute‐phase’ glycoproteins
- 17 May 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 322 (3), 266-276
- https://doi.org/10.1016/0014-5793(93)81584-m
Abstract
An intense broad resonance at 2.14 ppm present in high field (400, 500 and 600 MHz) Hahn spin-echo 1H-NMR spectra of rat blood plasma, but absent from those of human blood plasma is attributable to the presence of terminal O-acetylsialate sugars in the molecularly mobile carbohydrate side-chains of ‘acute-phase’ glycoproteins (predominantly α1-acid glycoprotein). The presence of such alternative acetylsugars in the carbohydrate side-chains of rat plasma glycoproteins are of much physiological and experimental significance in view of the regular use of these animals in model systems of human inflammatory conditions.Keywords
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