Experiments on Adsorption in Vitro of Type II Poliomyelitis Virus on Red Cells

Abstract

Human red cells from individual donors, when combined with poliomyelitis virus Type II, differ in their ability to remove virus from the supernatant and to anchor infectious doses on the cellular surface. This virus-removing property shows some relationship with the levels of type-specific neutralizing antibodies in the serum of the donor. Red cells from normal animals do not remove virus from the supernatants of virus-red cell mixtures, but virus removal may be demonstrated with cells from the same animals after their hyperimmunization with the specific antigen. Red cells which do not possess virus-removing capacity, whether from man or animals, acquire the property to remove virus after trypsinization of the erythrocytes. The mechanism of the virus-removing reaction is discussed.