Fink-Heimer Silver Impregnation of Degenerating Axons and Terminals in Mounted Cryostat Sections of Fresh and Fixed Brains
- 1 January 1970
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 45 (5), 199-204
- https://doi.org/10.3109/10520297009067479
Abstract
The silver impregnation method of Fink and Heimer (Brain Res., 4: 369-74, 1967) has been used on cryostat sections of both fresh frozen and formalin-fixed brain tissue mounted on slide. The fixed brains were soaked in 25% sucrose for 2-3 days before freezing. The slides used for mounting were dipped in a 0.5% aqueous gelatin solution containing 50 mg of chrome-alum per 100 ml, drained and allowed to dry in a vertical position. Sections of fresh tissue were fixed for 16 hr in a 10% formalin solution buffered with phosphates to pH 7.0. Staining was carried out according to the Fink-Heimer procedure II and gave results comparable to those obtained on unmounted frozen sections of formalin-fixed materialKeywords
This publication has 7 references indexed in Scilit:
- Silver impregnation of degenerating axons and their terminals on Epon-Araldite sectionsBrain Research, 1969
- Silver impregnation of terminal degeneration in some forebrain fiber systems: a comparative evaluation of current methodsBrain Research, 1967
- Two methods for selective silver impregnation of degenerating axons and their synaptic endings in the central nervous systemBrain Research, 1967
- An electron microscope study on the termination of the perforant path fibres in the hippocampus and the fascia dentataCell and tissue research, 1967
- Location and identification of excitatory synapses on hippoeampal pyramidal cellsExperimental Brain Research, 1966
- THE EXTRINSIC AFFERENT, COMMISSURAL AND ASSOCIATION FIBRES OF THE HIPPOCAMPUSBrain, 1965
- Terminal Degeneration within the Central Nervous System as Studied by a New Silver MethodJournal of Neuropathology and Experimental Neurology, 1946