Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication
- 1 September 1990
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 223 (2), 198-204
- https://doi.org/10.1007/bf00265054
Abstract
Chromosomes of Brassica oleracea (2n=18) were dissected from the resynthesized amphidiploid B. napus Hakuran by repeated backcrosses to B. campestris (2n=20), creating a series of monosomic alien chromosome addition line plants (2n=21). Using morphological, isozyme and restriction fragment length polymorphism markers (RFLPs), 81 putative loci were identified. Of nine possible synteny groups, seven were represented in the 25 monosomic addition plants tested. Sequences homologous to 26% of the 61 DNA clones utilized (80% were cDNA clones) were found on more than one synteny group, indicating a high level of gene duplication. Anomalous synteny associations were detected in four 2n=21 plants. One of these plants showed two markers from one B. oleracea chromosome associated with a second complete B. oleracea synteny group, suggesting translocation or recombination between non-homologous chromosomes in Hakuran or the backcross derivatives. The other three 2n=21 plants each contained two or more B. oleracea synteny groups, suggesting chromosome substitution.This publication has 28 references indexed in Scilit:
- Generation of alien chromosome addition lines from synthetic Brassica napus: morphology, cytology, fertility, and chromosome transmissionGenome, 1990
- Development and chromosomal localization of genome-specific DNA markers of Brassica and the evolution of amphidiploids and n = 9 diploid speciesGenome, 1990
- Coordinate expression of transcriptionally regulated isocitrate lyase and malate synthase genes in Brassica napus L.Plant Cell, 1989
- Spatial patterns of gene expression in Brassica napus seedlings: identification of a cortex-specific gene and localization of mRNAs encoding isocitrate lyase and a polypeptide homologous to proteinases.Plant Cell, 1989
- Structural and transcriptional characterization of the external spacer of a ribosomal RNA nuclear gene from a higher plantEuropean Journal of Biochemistry, 1988
- Nucleotide sequence of a cDNA clone of Brassica napus 12S storage protein shows homology with legumin from Pisum sativumPlant Molecular Biology, 1985
- Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. Oleracea ssp. capitata). V. The inheritance of resistanceEuphytica, 1983
- Chloroplast DNA evolution and the origin of amphidiploid Brassica speciesTheoretical and Applied Genetics, 1983
- Secondary Pairing in Brassica oleraceaCYTOLOGIA, 1937
- On the Chromosome Number of Brassica juncea and B. napus, on the Hybrid Between the Two, and an Offspring Line of the HybridThe Japanese Journal of Genetics, 1934