Continuous Flow Determination of Blood Alcohol Using Biamperometric Monitoring of Enzymatic Reaction
- 1 July 1987
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 20 (7), 1099-1113
- https://doi.org/10.1080/00032718708064594
Abstract
Ethanol was determined by reaction with NAD in the presence of alcohol dehydrogenase in a continuous flow system. NADH produced was allowed to react with hexacyanoferrate (III) in the presence of diapharase. The concentration of the produced hexacyanoferrate (II) was monitored biamperometrically using open tubular carbon electrodes. A level of 1.5 × 102 mg/100 ml ethanol was detected, with an average precision of 4% relative standard deviation. The ethanol level in blood samples was determined, obtaining a 0.08 correlation coefficient with gas chromatography results.Keywords
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