Direct analysis of rat bile for acetaminophen and two of its conjugated metabolites via thermospray liquid chromatography/mass spectrometry
- 1 December 1987
- journal article
- research article
- Published by Wiley in Journal of Mass Spectrometry
- Vol. 14 (12), 705-709
- https://doi.org/10.1002/bms.1200141203
Abstract
Bile from rats treated with acetaminophen was analyzed by direct injection onto a thermospray liquid chromatography/mass spectrometry (LC/MS) system. Two conjugated metabolites of acetaminophen were separated by the high‐pressure liquid chromatographic system and analyzed by mass spectrometry. The conjugates were identified as the glutathione–acetaminophen conjugate and the glucuronide–acetaminophen conjugate by comparison of the chromatographic retention times and the mass spectra to that of the synthetic standards. No evidence of acetaminophen metabolites was observed when bile samples were subjected to direct analysis by fast atom bombardment mass spectrometry.Keywords
This publication has 24 references indexed in Scilit:
- Glucuronic Acid, Sulfate Ester, and Glutathione Xenobiotic ConjugatesPublished by American Chemical Society (ACS) ,1986
- Structural characterization of the major covalent adduct formed in vitro between acetaminophen and bovine serum albuminChemico-Biological Interactions, 1985
- N-acetyl-p-benzoquinone imine: a cytochrome P-450-mediated oxidation product of acetaminophen.Proceedings of the National Academy of Sciences, 1984
- Comparison of six rabbit liver cytochrome P-450 isozymes in formation of a reactive metabolite of acetaminophenBiochemical and Biophysical Research Communications, 1983
- Evidence for the involvement of N-ACETYL-p-quinoneimine in acetaminophen metabolismBiochemical Pharmacology, 1979
- N-hydroxyacetaminophen: A microsomal metabolite of N-hydroxyphenacetin but apparently not of acetaminophenLife Sciences, 1979
- Determination of paracetamol and its metabolites in urine by high-performance liquid chromatography using reversed-phase bonded supportsJournal of Chromatography A, 1977
- Acetaminophen-induced Hepatic NecrosisPharmacology, 1974
- PLASMA-PARACETAMOL HALF-LIFE AND HEPATIC NECROSIS IN PATIENTS WITH PARACETAMOL OVERDOSAGEThe Lancet, 1971
- Acute liver necrosis following overdose of paracetamol.BMJ, 1966