Separation of porphyrin isomers by high-performance liquid chromatography
- 1 May 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 211 (2), 435-438
- https://doi.org/10.1042/bj2110435
Abstract
A reversed-phase gradient elution system is described for the simultaneous separation of the type I and type III isomers of 8-, 7-, 6-, 5- and 4-carboxylated porphyrins and isocoproporphyrins. The method, adaptable for isocratic and stepwise separation of individual groups of isomers, is also suitable for preparative isolation of pure porphyrins. The analyses of porphyrin isomers in the urine and feces of porphyric patients are examples of applications.This publication has 13 references indexed in Scilit:
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