INDUCTION OF THE DNA-REPAIR ENZYME URACIL-DNA GLYCOSYLASE IN STIMULATED HUMAN-LYMPHOCYTES
- 1 January 1979
- journal article
- research article
- Vol. 39 (6), 2090-2095
Abstract
The capacity of human cells to modulate the synthesis of DNA repair enzymes was investigated by measuring the induction of the uracil-DNA glycosylase during lymphocyte stimulation. Treatment of peripheral lymphocytes with phytohemagglutinin [PHA] increased glycosylase activity 10-fold. Glycosylase stimulation was coordinate with the activation of DNA synthesis and DNA polymerase activity. Two chromatographically distinct species of the glycosylase were resolved. Only 1 species was induced during PHA stimulation. The effect of actinomycin D and cycloheximide on glycosylase induction was determined. Treatment with either inhibitor at 96 h after PHA addition (maximal induction) decreased glycosylase activity after an appreciable lag period. Induction of the uracil-DNA glycosylase apparently requires transcription and translation although the enzyme may be quite stable once induced.This publication has 13 references indexed in Scilit:
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