α-DNA IX: parallel annealing of α-anomeric oligodeoxyribonucleotides to natural mRNA is required for interference in RNase H mediated hydrolysis and reverse transcription
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (13), 5107-5115
- https://doi.org/10.1093/nar/17.13.5107
Abstract
Ps- and aps-.alpha. anomeric oligodeoxyribonucleotides were designed to recognize in parallel (ps) or antiparallel (aps) orientation two different sites of a 1000 base-long mRNA. Northern blots experiments indicate that only ps-.apprx.-oligonucleotides were able to hybridize to the mRNA target. Furthermore, only ps-.alpha.-oligonucleotides were able, in a sequence specific way, to protect the mRNA target against RNase H mediated hydrolysis or to inactivate the priming capacity of .beta.-oligodeoxynucleotide probes in reverse transcription. Formation of parallel-stranded mRNA .alpha.-oligonucleotide miniduplexes which prevents hybridization of .beta.-oligonucleotide probes is the most likely mechanism accounting for these results. Use of .alpha.-oligonucleotides as potential gene control agents is discussed.This publication has 23 references indexed in Scilit:
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