THE EFFECT OF COLLINS' SOLUTION ON THE FUNCTION AND STRUCTURE OF ISOLATED PROXIMAL CONVOLUTED TUBULES FROM RABBIT KIDNEYS

Abstract

Proximal convoluted tubules (PCTs) from rabbitcortical slices were perfused after preservation in Collins' solution with the in vitro microperfuison technique. Under these conditions, after maneuves of in vitro preservation, the following findings were observed: (1) Tubules were best preserve, functionally and morphologically, when bated with Collins' solution peritubularly. (2) Tubular preservation was inadequate when the Collins' solution contacted only the luminal or luminal and peritubular sides. These observations indicate a difference in the reactions of various cellualr sides to the preseration process, suggeting that during preservaton of the whole kidney, there are differences in the preservation of the various tissues of the organ.