Growth of Parthenocissus tricuspidata tissue cultured on media containing aza analogues of indole-3-acetic acid & indole-3-propionic acid

Abstract

Normal and crown-gall tumor tissues were grown on media containing IAA and IPA and 2 aza analogues of these growth substances. Under the conditions of culture, IPA and the aza-IPA proved to be the most satisfactory for obtaining maximal growth. It appears that these compounds exert their effect on the growth of the cultures through an inhibition of IAA oxidase. The aza compounds are not simple metabolic antagonists of the natural auxin of these tissues. The tumor tissue is more sensitive to the effects of these compounds than is the normal tissue. The difference in the response of the 2 tissues can be explained most simply on the assumption that the intrinsic difference in the auxin requirements of the 2 tissues results from a difference in the balance between the auxin production and destruction and not from either of these factors taken separately.