Characterization of ouabain resistant, hypoxanthine phosphoribosyl transferase deficient human cells and their usefulness as a general method for the production of human cell hybrids
- 1 January 1980
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 28 (4), 227-239
- https://doi.org/10.1159/000131536
Abstract
Ouabain resistant mutants of hypoxanthine phosphoribosyl transferase deficient HeLa cells and euploid human fibroblasts were isolated and extensively characterized. These double mutants were used to test the prediction that they would be “universal hybridizers” for intraspecific human cell hybrid production. Hybrids were selected from fusions with human cells of diverse somatic origin. In addition it was shown that multi-parental hybrids can be generated using a modification of the selection system, a result which will be useful for gene dosage experiments involving human cells.This publication has 6 references indexed in Scilit:
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