Presynaptic Adenosine A2 and N‐Methyl‐D‐Aspartate Receptors Regulate Dopamine Synthesis in Rat Striatal Synaptosomes
- 1 May 1991
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 56 (5), 1783-1788
- https://doi.org/10.1111/j.1471-4159.1991.tb02081.x
Abstract
Dopamine synthesis rate and cyclic AMP concentration were measured in synaptosomes prepared from rat striatum. Dopamine synthesis rate was decreased by the addition of either adenosine deaminase or 8-phenyltheophylline, an adenosine receptor blocker, and was increased by the addition of 2-chloroadenosine. The addition of L-glutamate in the absence of adenosine deaminase decreased both dopamine synthesis rate and cyclic AMP concentration; in the presence of adenosine deaminase, glutamate had no effect on basal dopamine synthesis, but enhanced K(+)-stimulated synthesis. Both these effects of glutamate were abolished in Ca2(+)-free medium or in the presence of 2-amino-5-phosphonovalerate, an N-methyl-D-aspartate (NMDA) receptor blocker. In Mg2(+)-free medium with adenosine deaminase, glutamate enhanced both basal and K(+)-stimulated synthesis. These results suggest that dopaminergic terminals have A2 adenosine receptors, whose activation can stimulate dopamine synthesis by a cyclic AMP-dependent mechanism, and NMDA receptors, which modulate dopamine synthesis by a Ca2(+)-dependent mechanism.Keywords
This publication has 33 references indexed in Scilit:
- The effect of presynaptic receptor stimulation on adenosine 3′,5′-cyclic monophosphate concentrations in rat cortical synaptosomesNeuroscience Letters, 1986
- Mild chronic stress leads to desensitisation of presynaptic autoreceptors and a long-lasting increase in noradrenaline synthesis in rat cortical synaptosomesNeurochemistry International, 1986
- Adenosine receptor and beta-adrenoceptor stimulation increases noradrenaline synthesis in hippocampal synaptosomesNeurochemistry International, 1986
- Adenosine as a NeuromodulatorAnnual Review of Neuroscience, 1985
- Dual calcium-dependent protein phosphorylation systems in pancreas and their differential regulation by polymyxin BLife Sciences, 1984
- Phospholipid-sensitive Ca2+-dependent protein kinase: a major protein phosphorylation systemMolecular and Cellular Endocrinology, 1984
- Differential location of adenosine A1 and A2 receptors in striatumNeuroscience Letters, 1983
- Tyrosine hydroxylase activation in depolarized dopaminergic terminals—involvement of Ca2+ -dependent phosphorylationNature, 1983
- l-Glutamic acid, a neuromodulator of dopaminergic transmission in the rat corpus striatum☆Neurochemistry International, 1982
- ADENOSINE REGULATES VIA TWO DIFFERENT TYPES OF RECEPTORS, THE ACCUMULATION OF CYCLIC AMP IN CULTURED BRAIN CELLSJournal of Neurochemistry, 1979