Separation of the tryptic fragments of sarcoplasmic reticulum ATPase with high performance liquid chromatography
- 13 February 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 167 (1), 33-36
- https://doi.org/10.1016/0014-5793(84)80827-3
Abstract
The four tryptic fragments of the Ca 2+ - and Mg 2+ -dependent ATPase from sarcoplasmic reticulum were separated on a TSK-G 4000 SW preparative high performance liquid chromatography column in the presence of sodium dodecyl sulfate. Using atomic absorption spectroscopy, the analysis of the protein peaks demonstrated that the calcium binding sites with high affinities are located in the smallest fragment (A 2 ; 25 kDa).Keywords
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