Ultrastructural localization of tyrosine hydroxylase in rat nucleus accumbens
- 20 July 1984
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 227 (1), 92-103
- https://doi.org/10.1002/cne.902270110
Abstract
Immunocytochemical localization of tyrosine hydroxylase (TH) was used to determine the ultrastructural morphology and synaptic associations of catecholaminergic terminals in the nucleus accumbens of the rat. The brains were fixed by vascular perfusion with 4% paraformaldehyde and 0.2% glutaraldehyde. Coronal sections cut with a vibrating microtome were incubated with rabbit antiserum to TH then immunocytochemically labeled by the peroxidase‐antiperoxidase method. Immunoreactivity for the enzyme was found within unmyelinated axons and axon terminals. These terminals contained either all small clear or combined small clear and large dense core vesicles. Approximately 40% of the labeled terminals formed symmetric synapses with unlabeled proximal or distal dendritic shafts. The dendrites showed a sparse distribution of spines. Axosomatic synapses and axonal associations of the TH‐containing terminals also were detected. The recipient perikarya were usually 10–20 μm in diameter and contained an indented nucleus and abundant cytoplasm. The content of large dense vesicles and synaptic associations with somata and proximal dendrites suggest that a certain proportion of the TH‐containing terminals within the nucleus accumbens are morphologically distinct from catecholaminergic terminals within the dorsal striatum. These differences are discussed in relation to neuropeptides and functions of the dopaminergic mesolimbic and nigrostriatal pathways.Keywords
This publication has 57 references indexed in Scilit:
- Frequency specific effects of dopamine in the nucleus accumbensNeuroscience Letters, 1981
- Dopaminergic nerve endings visualised by high-resolution autoradiography in adult rat neostriatumNature, 1980
- Peptidergic neuronesNature, 1980
- Electrophysiological examination of the ventral tegmental (A10) area in the ratBrain Research, 1980
- A quantitative analysis of the excitatory and depressant effects of dopamine on the firing of caudatal neurons: electrophysiological support for the existence of two distinct dopamine-sensitive receptorsBrain Research, 1978
- Neurochemical changes following kainic acid lesions of the nucleus accumbens: Implications for a gabaergic accumbal-ventral tegmental pathwayLife Sciences, 1978
- GABAergic inhibition of neurons in the ventral tegmental areaCellular and Molecular Life Sciences, 1978
- Experimental demonstration of intrinsic synapses in cat's caudate nucleusNeuroscience Letters, 1977
- Autoradiographic tracing of nucleus accumbens efferents in the ratBrain Research, 1976
- The central adrenergic system. An immunofluorescence study of the location of cell bodies and their efferent connections in the rat utilizing dopamine‐B‐hydroxylase as a markerJournal of Comparative Neurology, 1975