Rapid Chromatography for Quantitation of Radioimmunoassayable 5α-Androstane-17β-OL-3-One and Testosterone in Ram, Bull and Boar Serum
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in Endocrine Research Communications
- Vol. 3 (1), 71-82
- https://doi.org/10.3109/07435807609057742
Abstract
A rapid technique for the simultaneous determination of serum 5alpha-androstane-17beta-0l-3-one (DHT) and testosterone (T) was developed and validated. The procedure incorporates a chromatographic step prior to binding with a T-3BSA antiserum. Anakrom columns used to separate DHT from T also isolate other steroids quickly and efficiently. Utilizing this procedure, levels of both androgens have been measured in the intact ram, bull and boar. Concentrations of DHT and T expressed as mean +/- SE in ng/ml were respectively: ram, 0.14 +/- 0.03 and 4.96 +/- 0.80; bull, 0.10 +/- 0.01 and 3.92 +/- 0.80; boar, 0.71 +/- 0.11 and 5.58 +/- 0.64. Whether these minute quantities of serum DHT play important physiological roles, particularly in the boar, is unresolved. Monitoring DHT under varying experimental or pathological conditions may allude to its importance in the domestic male.Keywords
This publication has 22 references indexed in Scilit:
- Effects of dihydrotestosterone and estradiol benzoate pretreatment upon testosterone-induced sexual behavior in the castrated male ratHormones and Behavior, 1975
- Methods for multiple steroid radioimmunoassayJournal of Steroid Biochemistry, 1975
- Actions of esters of testosterone, dihydrotestosterone, or estradiol on sexual behavior in castrated male guinea pigsHormones and Behavior, 1974
- Simultaneous radio immunoassay of testosterone and dihydrotestosteroneSteroids, 1974
- Mass spectrometric identification of testosterone, androstenedione, dehydroepiandrosterone, dihydrotestosterone, and androstanediol in human peripheral plasmaJournal of Steroid Biochemistry, 1973
- Serum Testosterone and Dihydrotestosterone Levels Following Orchiectomy in the Adult RatEndocrinology, 1973
- Determination of serum testosterone and androstanediol by competitive protein bindingSteroids, 1973
- SUPPRESSION OF LUTEINIZING HORMONE SECRETION IN MALE RATS BY 5α-ANDROSTAN-17β-OL-3-ONE (DIHYDROTESTOSTERONE) PROPIONATEJournal of Endocrinology, 1973
- Plasma 5α-dihydrotestosterone (17β-hydroxy-5α-androstan-3-one) in adolescent males at different stages of sexual maturationSteroids, 1972
- Selective Retention of Dihydrotestosterone by Prostatic NucleiNature, 1968