Abstract
A cytological study of Pyronema confluens has revealed that only one meiosis occurs in the ascus, and that the haploid chromosome complement is probably 12. Differences in size and staining properties were noted between nuclei of the ascogenous hyphae and paraphyses. Diplotene chromosomes are linearly differentiated into heterochromatic knobs and achromatic intercalary segments that could not be differentiated from the cytoplasm with either basic fuchsin or propiono-carmine.