THE UPTAKE OF GLUCOSE-6-T AND GLYCINE-2-T BY RHIZOCTONIA SOLANI KUHN

Abstract

The uptake of glucose-6-T and glycine-2-T by Rhizoctonia solani can be followed autoradiographically using a stripping film technique. In the young mycelium the nutrient is absorbed uniformly over the surface of the hyphae, but in older hyphae the uptake is unevenly distributed and related to the density of the cytoplasm. Competitive feeding experiments suggest that ribose, sucrose, and glycine enter the hypha by pathways other than that followed by the hexoses, glucose, mannose, galactose, and fructose, and that glycine enters by the same route as alanine, serine, and sodium glutamate. The effect of metabolic inhibitors on glucose uptake is the same as that found in mammalian tissue culture cells, and the distribution of radioactivity in the hyphae in the presence of dinitrophenol is consistent with the hypothesis that the uptake of glucose proceeds by a two-step process.